Rev. biol. mar. oceanogr. 48(3): 653-660Nota Científica |
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Inducción al desove de la navaja Ensis macha mediante inyección de serotonina |
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Daniel Arriagada1, Irene Lépez1, Maryori Ruiz2 e Ingrid Contreras1 |
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1Laboratorio Ingeniería Acuícola Irene Lépez Ltda., Gobernador Juan Henríquez 910, Concepción, Chile
2Programa de Doctorado Sistemas Marinos Costeros, Facultad de Recursos del Mar, Universidad de Antofagasta, Casilla 170, Antofagasta, Chile
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The effect of serotonin spawning stimulating agent, was evaluated in Ensis macha sexually mature broodstock, conditioned in laboratory. Neurotransmitter 0.9 ml at a concentration of 10-5 M was injected into the foot of each specimen. 5-HT induced gamete release in 92% of injected broodstock, always producing the fastest response in males, removing sperm at 36 min (± 15.9) after injection. The release of mature oocytes began at 170 min (± 42.4) post-injection. Embryonic development to the veliger larva formation, straight hinge D had a survival of 88%, no malformations have been observed in larvae. In only one trial, fertilized oocytes were not viable. Finally, we can confirm that there is a pattern in the release of mature gametes E. macha broodstocks due to the action of serotonin 5-HT and could be a useful tool for aquaculture practices.
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Key words: Ensis macha, serotonin, spawning |
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